Intermediate

Part:BBa_M1206:Design

Designed by: Andrea Olson   Group: UtahState_BE5930_-_S11   (2011-04-18)


Suicide Gene with GFP Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2158
    Illegal NheI site found at 2181
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1763
    Illegal AgeI site found at 1833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724
    Illegal SapI site found at 2434


Design Notes

Must consider lag time when transitioning from above 42C to below. It will take time for the repressor proteins to degrade once the coding for them stops when below 42C.


Source

Kill Gene Design Idea http://2010.igem.org/Team:HKU-Hong_Kong

References